chimeric monoclonal antibody  (Sino Biological)

Journal: medRxiv

Article Title: Diagnostic Classification for Long Covid Patients identifying Persistent Virus and Hyperimmune Pathophysiologies

doi: 10.64898/2026.04.21.26351402

Figure Lengend Snippet:

Article Snippet: The integrity of the spike protein samples on the surface was tested using an anti-S2 antibody (SinoBiological, 40590-D001), chosen for its specificity to a pan-variant conserved epitope.

Techniques:

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-SARS-CoV-2 induces binding antibodies on the nasal mucosa Heatmap depicting binding IgA (left), IgG (center) and IgM (right) responses observed in hamster nasal wash (top), BAL (center), and serum (bottom) for each immunization group (columns) against the indicated SARS-CoV antigens (rows), including Spike (S), the S2 and receptor-binding domain (RBD), and Nucleocapsid protein. Mean response values in each group of 6 animals are graphed.

Article Snippet: anti-SARS-CoV-2 spike monoclonal antibody (clone MM117) , Sino Biological Inc. , Cat#40592-MM117; RRID: AB_3075426.

Techniques: Binding Assay

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-Spike immunization limits weight loss following SARS-CoV-2 challenge Average percent body weight relative to the day of challenge, at either 56 days or 174 days following the first immunization. Hamsters were weighed once individually per day. Dots represent median and bars represent standard error for each group ( N = 10 hamsters).

Article Snippet: anti-SARS-CoV-2 spike monoclonal antibody (clone MM117) , Sino Biological Inc. , Cat#40592-MM117; RRID: AB_3075426.

Techniques:

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN immunization with Ad4-Anc reduces lung pathology following SARS-CoV-2 challenge Hamsters from each group were sacrificed four days after challenge (day 60 or 178) to assess disease severity in the lungs. Scores ranging from 1 to 5 were assigned based on pneumonia severity and IHC staining in the bronchial epithelium and alveoli. Average scores for each group ( N = 4 hamsters), and the percent of the lung affected by pneumonia, are indicated.

Article Snippet: anti-SARS-CoV-2 spike monoclonal antibody (clone MM117) , Sino Biological Inc. , Cat#40592-MM117; RRID: AB_3075426.

Techniques: Immunohistochemistry

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-Spike induces the durable restriction of SARS-CoV-2 replication after transmission. Weight loss and viral replication data following transmission at day 268 (A) Percent body weight relative to the day of challenge, represented as in . Dots represent the median for each group ( N = 6 hamsters) and bars represent standard error (B) AUC of viral copies measured in oropharyngeal swabs of individual hamsters, as represented in . Values for each hamster are indicated with dots, the median AUC value for each group ( N = 6 hamsters) is represented as a black line. The dotted line indicates the AUC limit of detection over 14 days (12,600 copies). (C) Curves representing viral replication over time after challenge. The limit of detection is depicted by the dotted line (10 copies per m L). Dots represent medians and bars represent standard error for each group ( N = 6 animals). The Wilcoxon rank-sum test was used for pairwise comparisons to generate the indicated p values.

Article Snippet: anti-SARS-CoV-2 spike monoclonal antibody (clone MM117) , Sino Biological Inc. , Cat#40592-MM117; RRID: AB_3075426.

Techniques: Transmission Assay

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-SARS-CoV-2 induces binding antibodies on the nasal mucosa Heatmap depicting binding IgA (left), IgG (center) and IgM (right) responses observed in hamster nasal wash (top), BAL (center), and serum (bottom) for each immunization group (columns) against the indicated SARS-CoV antigens (rows), including Spike (S), the S2 and receptor-binding domain (RBD), and Nucleocapsid protein. Mean response values in each group of 6 animals are graphed.

Article Snippet: For surface staining cells were harvested using 0.01 M EDTA in PBS 48 h after infection and stained with mouse anti-SARS-CoV-2 spike monoclonal antibody (Sino Biological, Inc., Beijing, China) (50 μL at 1.0 μg/mL final concentration), washed twice with phosphate buffered saline (PBS) (Quality Biological, Gaithersburg, MD, USA) containing 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), then stained with a goat anti-mouse IgG PE-conjugated secondary antibody (BioLegend, San Diego, CA, USA) (100 μL at 2.0 μg/mL final concentration) at 37°C for 30 min each.

Techniques: Binding Assay

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-Spike immunization limits weight loss following SARS-CoV-2 challenge Average percent body weight relative to the day of challenge, at either 56 days or 174 days following the first immunization. Hamsters were weighed once individually per day. Dots represent median and bars represent standard error for each group ( N = 10 hamsters).

Article Snippet: For surface staining cells were harvested using 0.01 M EDTA in PBS 48 h after infection and stained with mouse anti-SARS-CoV-2 spike monoclonal antibody (Sino Biological, Inc., Beijing, China) (50 μL at 1.0 μg/mL final concentration), washed twice with phosphate buffered saline (PBS) (Quality Biological, Gaithersburg, MD, USA) containing 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), then stained with a goat anti-mouse IgG PE-conjugated secondary antibody (BioLegend, San Diego, CA, USA) (100 μL at 2.0 μg/mL final concentration) at 37°C for 30 min each.

Techniques:

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN immunization with Ad4-Anc reduces lung pathology following SARS-CoV-2 challenge Hamsters from each group were sacrificed four days after challenge (day 60 or 178) to assess disease severity in the lungs. Scores ranging from 1 to 5 were assigned based on pneumonia severity and IHC staining in the bronchial epithelium and alveoli. Average scores for each group ( N = 4 hamsters), and the percent of the lung affected by pneumonia, are indicated.

Article Snippet: For surface staining cells were harvested using 0.01 M EDTA in PBS 48 h after infection and stained with mouse anti-SARS-CoV-2 spike monoclonal antibody (Sino Biological, Inc., Beijing, China) (50 μL at 1.0 μg/mL final concentration), washed twice with phosphate buffered saline (PBS) (Quality Biological, Gaithersburg, MD, USA) containing 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), then stained with a goat anti-mouse IgG PE-conjugated secondary antibody (BioLegend, San Diego, CA, USA) (100 μL at 2.0 μg/mL final concentration) at 37°C for 30 min each.

Techniques: Immunohistochemistry

Journal: iScience

Article Title: Intranasal replicating adenovirus type 4-vectored SARS-CoV-2 vaccines induce durable and efficacious responses in preclinical testing

doi: 10.1016/j.isci.2026.115433

Figure Lengend Snippet: IN Ad4-Spike induces the durable restriction of SARS-CoV-2 replication after transmission. Weight loss and viral replication data following transmission at day 268 (A) Percent body weight relative to the day of challenge, represented as in . Dots represent the median for each group ( N = 6 hamsters) and bars represent standard error (B) AUC of viral copies measured in oropharyngeal swabs of individual hamsters, as represented in . Values for each hamster are indicated with dots, the median AUC value for each group ( N = 6 hamsters) is represented as a black line. The dotted line indicates the AUC limit of detection over 14 days (12,600 copies). (C) Curves representing viral replication over time after challenge. The limit of detection is depicted by the dotted line (10 copies per m L). Dots represent medians and bars represent standard error for each group ( N = 6 animals). The Wilcoxon rank-sum test was used for pairwise comparisons to generate the indicated p values.

Article Snippet: For surface staining cells were harvested using 0.01 M EDTA in PBS 48 h after infection and stained with mouse anti-SARS-CoV-2 spike monoclonal antibody (Sino Biological, Inc., Beijing, China) (50 μL at 1.0 μg/mL final concentration), washed twice with phosphate buffered saline (PBS) (Quality Biological, Gaithersburg, MD, USA) containing 1% bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), then stained with a goat anti-mouse IgG PE-conjugated secondary antibody (BioLegend, San Diego, CA, USA) (100 μL at 2.0 μg/mL final concentration) at 37°C for 30 min each.

Techniques: Transmission Assay